Figure 5
From: Tumor resident memory CD8 T cells and concomitant tumor immunity develop independently of CD4 help
Decreased tumor-specific memory following anti-CD40L or anti-CD4 treatment at tumor implantation. (a) PK5L1940 pancreatic adenocarcinoma cells were injected sc. into C57BL/6 mice or C57BL/6 mice treated with anti-CD40L on d0, 1, and 2, or C57BL/6 mice treated with anti-CD4 on d-1. Control mice received no treatment. (b) Mice were treated with Lm-SIY on d21 and analyzed for SIY-specific T cells in the spleen on d28 by intracellular cytokine staining for IFNg following SIY peptide stimulation. (c) Quantitative analysis of SIY-specific cells in the spleen at d28.
