Table 1 Primers selected for phylogenetic analysis and inter and intraspecific diversity analysis of Ramulariopsis isolates.

Actin (ACT) ¹

ACT-512F

ATG TGC AAG GCC GGT TTC GC

Forward

Carbone & Kohn²⁶

58°-25’’/72°-25’’

ACT-783R

TAC GAG TCC TTC TGG CCC AT

Reverse

Carbone & Kohn²⁶

58°-25’’/72°-25’’

BOX²

BOXA1R

CTA CGG CAA GGC GAC GCT GAC G

–

Martin et al.²⁷

53°-60″/72°-8’

Elongation factor 1-α (EF1α)^1,3

EF1F1¹

TGC GGT GGT ATC GAC AAG CGT

Forward

Jacobs et al.²⁸

54°-45’’/72°-45’’

EF2R1¹

AGC ATG TTG TCG CCG TTG AAG

Reverse

Jacobs et al.²⁸

54°-45’’/72°-45’’

RG-TEF-F³

GTCCAACCCACGACAGCGACATC

Forward

This study

59°-45’’/72°-30’’

RG-TEF-R³

GTCTCCTTGATGATCTCGTTGTAGCGGT

Reverse

This study

59°-45’’/72°-30’’

RP-TEF-F³

TCGCCTAATCCATCAAACCGACACC

Forward

This study

59°-45’’/72°-30’’

RP-TEF-R³

GTCTCCTTGATGATCTCATTGTAGCGGT

Reverse

This study

59°-45’’/72°-30’’

RP-TEF-F2³

CCGACACCGATACCAACAATAATACC

Forward

This study

59°-45’’/72°-30’’

RG-TEF-R2³

GTCTCCTTGATGATCTCGTTGTAGCGGT

Forward

This study

59°-45’’/72°-30’’

RG-TEF-R3³

GTATATTTTTCTCCCATTGTCACA

Reverse

This study

59°-45’’/72°-30’’

RP-TEF-R2³

CCAACCCTCACGTGCAT

Reverse

This study

59°-45’’/72°-30’’

ERIC ²

ERIC1R

ATG TAA GCT CCT GGG GAT TCA C

Forward

Hulton et al.²⁹

52°-60″/72°-8’

ERIC2

AAG TAA GTG ACT GGG GTG AGC G

Reverse

Hulton et al.²⁹

52°-60″/72°-8’

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ¹

GPD1¹

CAA CGG CTT CGG TCG CAT TG

Forward

Berbee et al. ³⁰

54°-45’’/72°-45’’

GPD2₁

GCC AAG CAG TTG GTT GTG C

Reverse

Berbee et al. ³⁰

54°-45’’/72°-45’’

Histone H3 (HIS3) ¹

CYLH3F

AGG TCC ACT GGT GGC AAG

Forward

Crous et al.¹⁸

60°-45’’/72°-30’’

CYLH3R

AGC TGG ATG TCC TTG GAC TG

Reverse

Crous et al.¹⁸

60°-45’’/72°-30’’

IRAP ²

CIIRAP1

CGT ACG GAA CAC GCT ACA GA

–

Santos et al.³¹

57,5°-30″/72°-120”

CIIRAP2

AAT AAC GTC TCG GCC TTC AG

–

Santana et al.³²

55,4°-30″/ 72°-120”

CIIRAP4

CTT TTG ACG AGG CCA TGC

–

Santos et al.³¹

54,9°-30″/ 72°-120”

ISSR ²

(CAG)⁵

CAG CAG CAG CAG CAG

–

Rodrigues et al.³³

60°-45″/72°-90”

(GA)⁸

GAG AGA GAG AGA GAG A

–

Andrea & Xitlali³⁴

44°-45″/72°-90”

(GACAC)³

GAC ACG ACA CGA CAC

–

Weising et al.³⁵

48°-45″/72°-90”

(TGTC)⁴

TGT CTG TCT GTC TGT C

–

Rodrigues et al.³³

48°-45″/72°-90”

(GTG)⁵

GTG GTG GTG GTG GTG

–

Gente et al.³⁶

58°-45″/72°-90”

(GACA)⁴

GAC AGA CAG ACA GAC A

–

Gente et al.³⁶

50°-45″/72°-90”

(GATA)⁴

GAT AGA TAG ATA GAT A

–

Gente et al.³⁶

35°-45″/72°-90”

RNA polymerase II second largest subunit (RPB2) ¹

RPB2-5f.

GAY GAY MGW GAT CAY TTY GG

Forward

Liu et al.³⁷

54°-45’’/72°-45’’

7cR

CCC ATR GCT TGY TTR CCC AT

Reverse

Liu et al.³⁷

54°-45’’/72°-45’’

REP ²

REP1R-1

III ICG ICG ICA TCI GGC

Forward

Stern et al.³⁸

44°-60″/72°-8’

REP2-1

ICG ICT TAT CIG GCC TAC

Reverse

Stern et al.³⁸

44°-60″/72°-8’

Universal primer N21 ²

N21

GGA TCC GAG GGT GGC GGT TCT

–

Bulat et al.³⁹

55°-45″/72°-90”

Universal primer M13 ²

M13

GAG GGT GGC GGT TCT

–

Vassart et al.⁴⁰

50°-45″/72°-90”

28S nrRNA (LSU) e Internal transcribed spacer (ITS) ¹

V9G

TTA CGT CCC TGC CCT TTG TA

Forward

De Hoog & Ende⁴¹

53°-45’’/72°-45’’

LR5

ATC CTG AGG GAA ACT TC

Reverse

Vilgalys & Hester⁴²

53°-45’’/72°-45’’