Table 1 qPCR analysis of bone and muscle marker genes.
From: A CRISPR/Cas9-generated mutation in the zebrafish orthologue of PPP2R3B causes idiopathic scoliosis
Gene | Genotype | Rel. expression | SEM | p-value |
|---|---|---|---|---|
Target gene | ||||
ppp2r3b (primer set 1) | WT | 1 | 0.20566 | 0.7752 |
ppp2r3b (primer set 1) | ppp2r3b−/− | 0.91618 | 0.19554 | |
ppp2r3b (primer set 2) | WT | 1 | 0.20067 | 0.241 |
ppp2r3b (primer set 2) | ppp2r3b−/− | 0.68974 | 0.14055 | |
Muscle marker genes | ||||
mstnb | WT | 1 | 0.21493 | 0.0512 |
mstnb | ppp2r3b−/− | 0.42757 | 0.12753 | |
mylz2 | WT | 1 | 0.20609 | 0.1026 |
mylz2 | ppp2r3b−/− | 0.56843 | 0.11124 | |
smyhc1 | WT | 1 | 0.21205 | 0.782 |
smyhc1 | ppp2r3b−/− | 1.29499 | 1.00891 | |
stnnc | WT | 1 | 0.34582 | 0.5894 |
stnnc | ppp2r3b−/− | 1.93724 | 1.63087 | |
myhz2 | WT | 1 | 0.24932 | 0.8358 |
myhz2 | ppp2r3b−/− | 1.0712 | 0.22007 | |
tnni2 | WT | 1 | 0.28223 | 0.6054 |
tnni2 | ppp2r3b−/− | 0.8193 | 0.18237 | |
myog | WT | 1 | 0.2499 | 0.6072 |
myog | ppp2r3b−/− | 1.18719 | 0.2449 | |
tnnc1b | WT | 1 | 0.25356 | 0.2269 |
tnnc1b | ppp2r3b−/− | 0.64582 | 0.09455 | |
Bone marker genes | ||||
ctsk | WT | 1 | 0.17927 | 0.16 |
ppp2r3b−/− | 1.81554 | 0.49503 | ||
rank l | WT | 1 | 0.2353 | 0.6865 |
ppp2r3b−/− | 1.15204 | 0.27555 | ||
col2a1a | WT | 1 | 0.19301 | 0.2527 |
ppp2r3b−/− | 1.61816 | 0.4685 | ||
mmp2 | WT | 1 | 0.2274 | 0.7332 |
ppp2r3b−/− | 0.89729 | 0.18151 | ||
mmp9 | WT | 1 | 0.27651 | 0.2306 |
ppp2r3b−/− | 0.61384 | 0.11011 | ||
mmp13 | WT | 1 | 0.3806 | 0.5372 |
ppp2r3b−/− | 1.36542 | 0.42002 | ||
timp2a | WT | 1 | 0.19044 | 0.1307 |
ppp2r3b−/− | 0.60948 | 0.13231 | ||
spp1 | WT | 1 | 0.17135 | 0.6068 |
ppp2r3b−/− | 1.1612 | 0.24748 | ||
runx2b | WT | 1 | 0.17498 | 0.8269 |
ppp2r3b−/− | 1.05905 | 0.19411 | ||
sox9b | WT | 1 | 0.15355 | 0.5506 |
ppp2r3b−/− | 1.16335 | 0.21253 | ||
