Figure 7

HF Diet Induces Liver Inflammation. The percentage of (A) CD3⁺CD4⁺, (B) CD3⁺CD8⁺ T cells and (C) M1 macrophages (F4/80⁺CD11c⁺) in liver mononuclear cells were detected by flow cytometry at 8 weeks. The cells were analyzed by FACS. (D) mRNA expression levels of the TNF-α, IL-6, and IL-1β were measured by RT-qPCR at 8 weeks. (E) Liver MDA activity was measured at 4, 8 and 12 weeks. (F) mRNA expression levels of the FAS, ACC1, CD36, ChREBP, and SREBP1c were measured by RT-qPCR. (G) Representative images of Western blots for lipid metabolism (FASN, ACC1, CD36, ChREBP, and SREBP1c). (H) Gray-scale value analysis of FASN, ACC1, CD36, ChREBP, and SREBP1c. The student’s t-test was used to determine statistical significance. *p < 0.05; **p < 0.01; ***p < 0.001.