Figure 8

The effect of FMT on insulin resistance, liver histology, and immune homeostasis imbalance in HF-fed mice. (A) Glucose tolerance test (left) and glucose tolerance test calculated AUC (right) measured after FMT treatment. (B) Insulin tolerance test (left) and insulin tolerance test calculated AUC (right) measured after FMT treatment. Representative images of liver stained with (C) H&E and (D) Oil-red-O. Bar: 50 µm. (E) Flow cytometry analysis of CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells, and M1 macrophages (F4/80⁺CD11c⁺) in liver. (F) Flow cytometry analysis of the MFI expression level of Th1 cells (CD4⁺IFN-γ⁺) and Treg cells (CD25⁺Foxp3⁺) in MLN. The levels of TNF-α (G), IL-1β (H) and IL-6 (I) in serum were determined using ELISA. One-way analysis of variance (ANOVA) test was used for determining statistical significance. *p < 0.05; **p < 0.01.