Figure 4
From: Arntl deficiency in myeloid cells reduces neutrophil recruitment and delays skeletal muscle repair
Arntl deletion from myeloid cells decreases expression of Cxcr2 related to neutrophil migration and reduces monocyte numbers in bone marrow and peripheral blood. (a) RT-PCR assessment of chemokine (C-X-C motif) receptor 2 (Cxcr2) expression in neutrophils (CD45+ CD11b+ Ly6G+) sorted from untreated bone marrow cells from Ctrl (n = 5) and cKO (n = 4) mice. (b) Percentage of CXCR2+ cells among Ctrl (n = 3) and cKO (n = 3) bone marrow CD45+ CD11b+ Ly6G+ neutrophils. (c) Numbers per field of migrated Ctrl (n = 3) and cKO (n = 3) neutrophils left untreated or stimulated with 100 ng/ml CXCL2. (d) Quantification of monocytes (CD45+ CD11b+ Ly6G- Siglec-F- Ly6Chi) among control (Ctrl) and cKO bone marrow cells from mice left untreated (n = 5) and on day 2 after muscle injury (n = 3). (e) Quantification of monocytes in peripheral blood from Ctrl and cKO mice left untreated (n = 5) and on day 2 after muscle injury (n = 3). Data are expressed as the means ± SEM. * p < 0.05 with unpaired two-tailed Student’s t-test (a, b, d, e) or two-way ANOVA with Bonferroni’s multiple comparisons test (c).
