Figure 4
Aberrant PI3-K signalling in Pten-knockout neural cells. Representative bright-field images of primary cultures of (A) neural progenitor cells (NPCs), (D) astrocytes (ASTs) and (G) neurons at DIV14. Scale bar = 0.1 mm. Red arrowheads, hypertrophic neurites. Immunoblotting of the indicated proteins in (B) NPCs, (E) ASTs and (H) neurons of Pten-knockout (Nes-KO) and littermate control (Ctrl) mice. Cortical lysate from a wild-type mouse (Ctx) was used as a control. The specific phosphorylation of p-AKT and p-GSK3-β relative to control (Ctrl) are shown in (C) NPCs, (F) ASTs and (I) PCN. An unpaired Student’s t-test was used for statistical analysis with the indicated p-values: * < 0.05, ** < 0.01, *** < 0.001 and **** < 0.0001.
