Figure 3
From: HDAC1 and HDAC6 are essential for driving growth in IDH1 mutant glioma
HDAC1 and HDAC6 are essential for growth in a panel of IDH1 mutant lines. (A) A panel of 10 surgical specimens (5 IDH1 wildtype and 5 IDH1 mutant) were stained with antibodies against HDAC proteins (HDAC1-7) and scored on a scale of 1–3 by a blinded neuropathologist. (B) A collection of 58 patient-derived cell lines (53 IDH1 wildtype, 5 IDH1 mutant) underwent expression analysis and the relative expression of each HDAC gene is shown. (C) Knock-down lentiviral ventors were designed against the 6 HDAC genes that were expressed in IDH1 mutant glioma tissue and cell lines (HDAC1-4, HDAC6, HDAC9) and tested on three IDH1 mutant lines. Relative growth was measured on day 14.
