Figure 4
From: HDAC1 and HDAC6 are essential for driving growth in IDH1 mutant glioma
HDAC enzymes up-regulate DNA replication gene modules. HDAC6 targets a unique set of gene modules. (A) Each HDAC knock-down underwent RNA-seq analysis. The number of up-regulated and down-regulated genes are shown. (B) These genes underwent gene set enrichment analysis (Panther) the most enriched modules for each knockdown are shown. (C) A list of significantly changed (> 2 fold changed) genes from each of the drug treatment data sets was assigned to one of the following sub-groups: (1) unique to one of the HDAC knockdown sets, (2) “non-unique” i.e. shared by more than one HDAC knock-down gene set, or (3) “unaccounted” if the gene was not found in any of the HDAC knock-down sets). (D) RNA-seq datasets from each knockdown as well as drug treatment samples (1 mM VPA, and 15 nM LBH) underwent PCA analysis (HK252).
