Figure 5

Biodistribution of the CoQ₁₀-MITO-Porter in mice. Ex vivo images of organs are obtained from non-treated or APAP-treated mice 3 h after injection with DiD-labeled CoQ₁₀-MITO-Porter. (A) Amount transferred to several main organs. Based on the observed images, fluorescence intensity is quantified using image J and defined as the amount of transferred to the organs. Data represent the mean ± S.D. (n = 3). Significant differences were calculated by the Unpaired t-test (**p < 0.01). (B) Transfer rate on several main organs. The transfer rate is calculated from the transfer amount results. Data represent the mean ± S.D. (n = 3). The significant differences (vs Liver) were calculated by one-way ANOVA, followed by SNK test (**p < 0.01). (C) Biodistribution image on several main organs. (a) Non-treatment. (b) APAP-treatment. Table S6 shows the physical properties of the administered CoQ₁₀-MITO-Porter.