Table 1 Primers used in this study and their characteristics.
Primer name | (5′–3′) primer sequence | Accession number | Length (bp) | Annealing temperature | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
SSR | AGGTGGGAGATATGCTAGAATGGG | CATTCTGTGTGTCTCCCTGCC | MK060115.1 | 152 | 61 |
SMT | GAGAAGGCTGCAGAGGGTCTAG | CACAGTAACTCATGACAACAGCAACCT | MK060114.1 | 140 | 62 |
SEP | CTATTCTCAGAAGGACCGATCTC | CAGATGCACCAGAGAGTAATCG | KX14846 | 168 | 60 |
CAS | AAGAGAGATCCAACACCACTGC | TACATGACGACGGTATTCTCCC | KX148475.1 | 17 | 60 |
SQS | GGCTCAACCATGATTCTCATACTG | TACCCACTGTTCCATTGCTATCC | KX148475.1 | 133 | 61 |
GAPDH (reference gene) | ATGTTAAATGATGCAGCCCTTCCACCTCTC | TATGTTTGTTGTTGGTGTCAACGAGCAACGAATACAAG | XM_010679634.2 | 230 | 61 |
