Figure 3
From: Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity
Chronic hepatic overexpression of hHKDC1 up-regulates the transcription factor YAP. (a) Immunoblot of Beta-catenin normalized to GAPDH and corresponding quantification. (b) Immunoblot of yes-associated protein (YAP) normalized to GAPDH and corresponding quantification, immunoblot of phosphorylated YAP at serine 127 (pYAP) normalized to GAPDH and corresponding quantification of the ratio between pYAP to total YAP. (c) Immunoblot of connective tissue growth factor (CTGF) normalized to GAPDH and corresponding quantification. (d) Immunoblot of cysteine-rich angiogenic inducer 61 (CYR61) normalized to GAPDH and corresponding quantification. n = 4–5 mice/group for (a–d). (e) Immunofluorescence microscopy of liver sections; Red: YAP, Blue: DAPI, n = 3 mice/group. All values are means ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 (Student t-test).
