Figure 4
From: Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity
Up-regulation of YAP, cell size, and proliferative capacity is moderately dependent on HKDC1 expression. (a) mRNA expression of hHKDC1 and (b) PCNA in the stable OE of hHKDC1 in AML-12 cells and stable OE of hHKDC1 with siRNA against HKDC1 normalized to β-actin relative to null, n = 3–4. (c) Immunoblots of YAP normalized to GAPDH and corresponding quantification. (d) Immunofluorescence microscopy of WGA stain of stable OE of null AML-12 cells, stable OE of hHKDC1, and stable OE of hHKDC1 with siRNA against HKDC1 and corresponding size quantification, n = 3, > 50 cells each. (e) Immunofluorescence microscopy of WGA stain of null AML-12 cells, cells with siRNA against Yap, stable OE of hHKDC1, and stable OE of hHKDC1 with siRNA against Yap and corresponding size quantification; n = 3, > 25 cells each; Green: WGA, Blue: DAPI and corresponding area of measurements. All values are means ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 (One-way ANOVA).
