Figure 5
From: Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity
Chronic hepatic overexpression of HKDC1 does not impact other hexokinases but causes an increase in HK activity and decreases in PKM2 and lactate. (a) mRNA expression of mouse Hkdc1, Hk1, Hk2, Hk3 and Gck in hHKDC1 OE and null mouse livers relative to null, n = 4–5 mice/group. (b) Immunoblots of hexokinase 1 (HK1), hexokinase 2 (HK2), hexokinase 3 (HK3) and glucokinase (Gck) normalized to corresponding GAPDH of hHKDC1 OE and null mouse livers. (c) Immunoblots of HKDC1, GCK, GAPDH (cytosolic housekeeping), and translocase of the outer mitochondrial membrane complex subunit 20 (TOM20, mitochondrial housekeeping) on mitochondrial (mito) and cytosolic (cyto) fractions of mouse livers and corresponding quantification of GCK. (d) Glucose-6-phosphate levels, (e) Hexokinase activity and (f) Lactate levels in mouse liver tissue, n = 4–5 mice/group. (g) Immunoblots of PKM2 and GAPDH. All values are means ± SEM. *P < 0.05 (Student t-test).
