Table 8 The sequences (5′–3′) of primers and probes for IL1B three htSNPs examined.

From: Common variants of pro-inflammatory gene IL1B and interactions with PPP1R13L and POLR1G in relation to lung cancer among Northeast Chinese

rs Number

Primers

Probes

rs1143633

 Forward primer

CTACTGGTGTTGTCATCAGAC

 

 Reverse primer

AGCTTTTTTGCTGTGAGTCCC

 

 Common probe

 

-P-CCTCGCCTCACGAGGCCTGCCCTTC-HEX-

 Discriminating probe G

 

TTTTCCTCCAAGAAATCAAATTTTGCCG

 Discriminating probe A

 

TCCTCCAAGAAATCAAATTTTGCCA

rs3136558

 Forward primer

TCGCCATGTTGGCCAGGCTGG

 

 Reverse primer

GTGGTCCTGCCAGGAACCATG

 

 Common probe

 

-P-CTTTAGAAGCTCGGGATTCTTTCAATTT-HEX-

 Discriminating probe C

 

TTTTCACGCCTGGCCCAGAGAGGGATGAC

 Discriminating probe T

 

TTTTTTTCACGCCTGGCCCAGAGAGGGATGAT

rs1143630

 Forward primer

GAATAGCCTGTAAGGTGTCAG

 

 Reverse primer

GGATGCAGTAAGCCAAGATTG

 

 Common probe

 

-P-CTTAACCTCCTTGAGCTTCAGAGAGTTTTTTTTT-FAM-

 Discriminating probe A

 

TTTTTTTTTTTTCCTGCTGTGTGCCCTTGAGTACACA

 Discriminating probe C

 

TTTTTTTTTTTTTTTCCTGCTGTGTGCCCTTGAGTACACC

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