Table 1 Accuracy assessment: comparison of sequencing results in the context of an EQA round trial organized by the NRC for two samples typed by our lab and by two independent labs.
Testing lab | Laboratory 1 | Laboratory 2 | Laboratory 3 | Laboratory 4 | CHU UCL Namur | CHU UCL Namur | CHU UCL Namur | |
|---|---|---|---|---|---|---|---|---|
Extraction method | N. A. | Seegene- STARMag Universal Cartridge (Allplex SARS-CoV-2 One-Step Protocol) | N. A. | Maxwell RSC Viral-Total Nucleic Acid Purification kit /MaxWell RSC-system (Promega) | MagMAXTM Viral/Pathogen Kit (KingFisherTM) | MagMAXTM Viral/Pathogen Kit (KingFisherTM) | Seegene- STARMag Universal Cartridge (Allplex SARS-CoV-2 One-Step Protocol) | |
Sequencing Method | ONT | Illumina CleanPlex SARS-CoV-2 Flex Panel-SOPHiADDM | Ampliseq- Illumina | ONT-GridION Artic Network | ONT: SARS-CoV2 genome sequencing protocol v1200 “midnight” | ONT: SARS-CoV2 genome sequencing protocol v3 | Illumina: EasySeq SARS-Cov-2 WGS-Nimagen | |
Sample UZL_ CV10_07 | WHO | Alpha | Alpha | – | – | Alpha | Alpha | Alpha |
NextClade | 20I | 20I | – | – | 20I | 20I | 20I | |
Pangolin | B.1.1.7 | B.1.1.7 | – | – | B.1.1.7 | B.1.1.7 | B.1.1.7 | |
Sample UZL_ CV10_89 | WHO | – | – | Delta | Delta | Delta | Delta | Delta |
NextClade | – | – | 21J | 21J | 21J | 21J | 21J | |
Pangolin | – | – | AY.39 | B.1.617.2 | AY.126 | AY.126 | AY.126 | |
