Table 1 Categorization of pollen grains into different morphological subtypes.

From: Evaluation of methods to assess the quality of cryopreserved Solanaceae pollen

Category

Description

Example image

Aberrant

Aberrant grains have been associated with abortion of the microspore development, e.g. due to heat stress29,30

Dehydrated

Pollen failing to morphologically reconstitute after rehydration and suspension in the germination buffer. Furrows can clearly be identified, resembling a “coffee bean”. See Fig. 1a–I

Reconstituted, not germinated

This category comprises pollen grains which were morphologically reconstituted by rehydration and suspension in germination buffer but did not form a pollen tube. See Fig. 1a–II

Reconstituted, pollen tube emerging

These pollen grains formed a pollen tube, but the tube length was shorter than the pollen’s diameter

Reconstituted, pollen tube longer than pollen diameter

These pollen grains formed pollen tubes with tubes longer than the pollen diameter

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