Table 2 RT-qPCR amplification parameters for Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit (Liferiver), Vitassay qPCR SARS-CoV-2 (Vitassay) and TaqPath COVID‑19 CE-IVD RT-PCR Kit (Thermo Fisher Scientific).
From: Commercially available SARS-CoV-2 RT-qPCR diagnostic tests need obligatory internal validation
Efficiency (%) | R2 | y-intercept | Mean Ct (SD) for 106 – 101 viral RNA copies per reaction | ||||||
|---|---|---|---|---|---|---|---|---|---|
106 | 105 | 104 | 103 | 102 | 101 | ||||
Liferiver | 92 | 0.999 | 39 | 18.251 (0.045) | 21.640 (0.135) | 25.226 (0.171) | 28.612 (0.242) | 32.306 (0.143) | 35.794 (0.173) |
Vitassay | 103 | 0.996 | 40 | 20.847 (0.220) | 24.041 (0.069) | 27.440 (0.207) | 30.606 (0.174) | 34.388 (0.120) | 36.724 (0.421) |
TaqPath | 100 | 0.998 | 36 | 16.298 (0.162) | 19.509 (0.423) | 23.130 (0.119) | 26.584 (0.163) | 29.495 (0.144) | 32.858 (0.201) |
