Table 3 Sensitivity of the studied RT-qPCR assays determined by analysis of twenty replicates of samples representing 1–100 copies of viral RNA per reaction.

From: Commercially available SARS-CoV-2 RT-qPCR diagnostic tests need obligatory internal validation

 

Number of positive results (N = 20)

1 copy/rxn

10 copies/rxn

100 copies/rxn

Liferiver

10 (mean Ct = 37.272)

20 (mean Ct = 35.476)

20 (mean Ct = 32.283)

Vitassay

5 (mean Ct = 38.758)

18 (mean Ct = 37.218)

20 (mean Ct = 34.307)

TaqPath

7 (mean Ct = 36.035)

20 (mean Ct = 32.971)

20 (mean Ct = 29.586)

  1. Mean Ct values corresponding to the respective copy numbers are given in brackets.