Figure 1
(A) Phylogenetic analysis of EfAmi1. Multiple sequence alignment was achieved using Cobalt37. The phylogenetic tree was constructed with Phylip38 and displayed with iTOL v439. (B) Multiple sequence alignment of NALAA homologous sequences with EfAmi1. Alignment was performed using ClustalΟ40 and displayed using ESPript 3.041. The NALAA sequences used (NCBI Accession number) were the following: WP_086274872.1 (EfAmi1); WP_259741559.1 (from Enterococcus faecium); WP_086299721.1 (from Enterococcus faecium); WP_149558365.1 (from Enterococcus faecium); WP_123837729.1 (from Enterococcus faecium]); UVX36018.1 (from Bacteriophage sp); UVY16197.1 (from Bacteriophage sp); YP_009042672.1 (from Enterococcus phage IME-EFm1); YP_009200901.1 (from Enterococcus phage IME-EFm5); QOI68841.1 (from Enterococcus phage 9184). The zinc-binding residues are shown with stars. EfAmi1 numbering is shown above the alignment. Conserved areas are shown shaded. A column is framed, if more than 70% of its residues are similar according to physico-chemical properties.
