Figure 3
Turbidity reduction assays. (A) Visual inspection of the reduction of the turbidity of the E. faecium deactivated cells after treatment with recombinant EfAmi1 (100 μg, 2.7 μM) for 120 min at 25 °C. (B) Enzyme lytic activity was determined by measuring the reduction of OD600nm using E. faecium cells as substrate. EfAmi1 activity was measured in the absence (blue line) or in presence of Zn2+ ion (1 mM, red line). Control reactions (without enzyme) in the presence and absence of Zn2+ ion (black and green lines, respectively) were also recorded. (C) Effect of pH in EfAmi1 activity. All assays were performed using turbidity measurements (OD600nm) in each pH value using E. faecium cells as substrate.
