Figure 2
From: Pancreatic β-cell glutaminase 2 maintains glucose homeostasis under the condition of hyperglycaemia
The effect of GLS2 on insulin from β-cells and glucagon from α-cells. (A) and (B), Immunohistochemical analysis of Gls2 (shown in brown) in pancreas from Gls2 CKO and RIP-Cre mice. Representative images in A. Scale bars: 20 μm. The relative Gls2 area is shown in dot plots in B. (C–E), Hematoxylin and eosin (H&E) staining and immunohistochemical analysis of insulin and glucagon in the pancreas from Gls2 CKO and RIP-Cre mice fed a HFD for 32 weeks were performed. The percentage of insulin-positive β-cells per islet (C) and the percentage of glucagon-positive α-cells per islet (D) were calculated. ***P<0.001. Representative images of the pancreas from HFD-fed Gls2 CKO and RIP-Cre mice at 32 weeks of age that were stained with hematoxylin and eosin (H&E), insulin antibody (shown in brown), and glucagon antibody (shown in brown) in E. Scale bars: 20 μm. (F–I), The pancreatic β-cell line MIN6 was transfected with either control (siCont) or siGls2 for 36 hours, and then Gls2 protein level (F), Gls2 gene expression using RT–qPCR (G), intracellular ATP levels (H) and insulin secretion from the cells to the medium (I) were determined. The blot was cut at 30 kDa before hybridization with β-actin antibody. Data are shown as the mean ± SEM. *P<0.05.
