Figure 5
From: Adenylyl cyclase 6 plays a minor role in the mouse inner ear and retina
Expression of adenylyl cyclase genes in mouse retinas. (a) RT-PCR analysis of AC1-AC10 mRNA expressions in wild-type and Rd1 mouse retinas at 2 months of age. M, DNA ladder. The original gel is presented in Supplementary Fig. S4. (b) Similar immunostaining signals from the AC5/AC6 antibody in adult wild-type (left) and Adcy6−/− (middle) mouse retinas. Compared with the negative control immunostaining without the primary antibody (right), the signals are present in the photoreceptor cell body, but not outer segment. The signals are also present in the inner retina. RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; Hoechst 33,342, nuclear dye; DIC, differential interference contrast; scale bar: 10 µm.
