Table 1 Primary antibodies used in this study.
From: Adenylyl cyclase 6 plays a minor role in the mouse inner ear and retina
Name | Cat # | Company | Host & Type | Validation | Concentration/dilution ratioc |
|---|---|---|---|---|---|
ADGRV1a | N/A | N/A | Rabbit polyclonal | Immunostaining in Adgrv1−/− inner ears30 | IF: 1:4000 |
AC5/6 | sc-590 | Santa Cruz | Rabbit polyclonal (C-17) | Immunostaining in Adcy6−/− inner ears (this study) | IF: 2 mg/ml WB: 0.2 mg/ml |
Gαi2 | sc-13534 | Santa Cruz | Mouse monoclonal (L5) | Immunoblot analysis in transfected HEK293 cells (this study) | IF: 2 mg/ml WB: 0.2 mg/ml |
Gαs | sc-823 | Santa Cruz | Rabbit polyclonal (K-20) | Immunoblot analysis in transfected HEK293 cells (this study) | IF: 2 mg/ml WB: 0.2 mg/ml |
γ-actin | sc-65634 | Scant Cruz | Mouse monoclonal (2–4) | Immunostaining and immunoblot analyses in Actg1−/− tissues57 | WB: 0.1 mg/ml |
Pan-PKA Cα | 4782 | Cell signaling | Rabbit polyclonal | Immunoblot analysis in siRNA knockdown Hela cells (by seller) | IF: 1:100 WB: 1:1,000 |
Phospho-PKA Cα (Thr198)b | 4781 | Cell signaling | Rabbit polyclonal | Immunoblot analysis of phosphatase-treated C6 and 3T3 cells (by seller) | IF: 1:100 WB: 1:1,000 |
Pan-Akt | 4691 | Cell signaling | Rabbit monoclonal (C67E7) | Immunostaining of insulin-treated C2C12 cells and immunoblot analysis of recombinant Akt proteins (by seller) | IF: 1:100 WB: 1:1,000 |
Phospho-Akt (Ser473) | 4060 | Cell signaling | Rabbit monoclonal (D9E) | Immunostaining and immunoblot analyses of various LY294002-treated and PDGF-treated cultured cells; Immunoprecipitation of Jurkat extracts using non-specific and specific antibodies (by seller) | IF: 1:100 WB: 1:1,000 |
FLAG tag | F1804 | Sigma | Mouse monoclonal (M2) | Immunoblot analysis in transfected HEK293 cells (this study) | WB: 0.3 mg/ml |
γ-tubulin | T6557 | Sigma | Mouse monoclonal (GTU-88) | Immunoblot analysis in cultured cells by skipping the primary antibody (by seller) | WB: 1:10,000 |
