Figure 2
From: Small RNA transcriptome analysis using parallel single-cell small RNA sequencing
Small RNA profiling of cultured cells via PSCSR-seq. (A) Distribution of small RNA molecules in A549 cells. The pie chart shows the proportion of small RNA molecules mapped to each RNA type (also see Supplementary Table S1). (B) Violin plots depicting the distribution of total small RNA molecule counts (scored by UMIs) per cell, miRNA species numbers per cell, and the total small RNA species numbers per cell. (C) tSNE projection of 1,145 A549 cells calculated from the expression profiles of miRNA and other small RNA forms. The proportion of particular small RNA forms among the total molecules in each cell is overlaid on each tSNE plot. Additionally, see Supplementary Fig. S6F. (D) tSNE projection of mixed cells calculated from the expression profiles of miRNA and other small RNA forms. Here, HeLa (n = 356), A549 (n = 242), K562 (n = 120), and HEK293T (n = 109) cells were used for the plots. Additionally, see Supplementary Fig. S6G.
