Figure 3
From: Mitochondrial citrate metabolism and efflux regulate BeWo differentiation
Loss of CIC impairs biochemical differentiation. (A) Representative western blot image of CIC and total protein expression in empty-vector, non-targeting, and CIC knockout BeWo cells treated cells. Please see Supplemental Fig. 4 for images of uncropped blots. (B) ELISA of HCG production in empty-vector, non-targeting, and CIC knockout BeWo cells treated with DMSO or 40 µM Forskolin (FSK). n = 3 biological replicates. (C) qPCR analysis of CGA, CGB2, ERVW-1, and ERVFRD-1 gene expression in empty vector, non-targeting, and CIC knockout BeWo cells treated with DMSO or 40 µM Forskolin (FSK). n = 3 biological replicates. Data are representative of mean +/− SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001.
