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Figure 2

From: In vitro and in vivo susceptibility to sulfadiazine and pyrimethamine of Toxoplasma gondii strains isolated from Brazilian free wild birds

Figure 2

Cystogenesis rate of Wild2, Wild3 and Wild4 in vitro after treatment with SDZ and PYR for 24 and 48 h. Treatment of intracellular parasites were initiated after 24 h of infection and labeled with SAG1 antibody for tachyzoite surface and DBA-rhodamine for cyst wall after 24 and 48 h treatment. SAG—vacuoles only containing tachyzoites; DBA—vacuoles only containing bradyzoites; SAG + DBA—vacuoles positive for DBA containing SAG-positive parasites (intermediate stage). Results represent the mean ± standard deviation of two independent experiments *P < 0.05; **P < 0.01.

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