Figure 4
From: Atretic preovulatory follicles could be precursors of ovarian lutein cysts in the pig
The expression of PTGS2 (A,B), PTGES (C) in follicular (17PF, 19-20PF, HPF, APF) and cystic (IPC, SOC) walls. Gene expression was normalized to the geometric mean of ACTB and GAPDH (AU), identified as the best reference genes by NormFinder algorithm. Protein levels were normalized to total protein content (AU) using TGX Stain-Free gel technology (B). Uncropped blots are presented in Supplementary Fig. S7 online. Data are presented as mean ± SEM. Data were analyzed using one-way ANOVA and LSD test by selecting appropriate planned comparisons. Letters (a, b) indicate a significant difference between groups (p < 0.05). For framed groups (follicles derived from ovaries of the same gilts), Student’s t-test was performed. AU arbitrary units.
