Figure 8
From: Atretic preovulatory follicles could be precursors of ovarian lutein cysts in the pig
The expression of TNFα mRNA (A) and concentration of TNFα (B) in follicular (17PF, 19-20PF, HPF, APF) and cystic (IPC, SOC) walls/fluid. Gene expression was normalized to the geometric mean of ACTB and GAPDH (AU), identified as the best reference genes by NormFinder algorithm. Data are presented as mean ± SEM. Data were analyzed using one-way ANOVA and LSD test by selecting appropriate planned comparisons. Letters (a, b) indicate a significant difference between groups (p < 0.05). For framed groups (follicles derived from ovaries of the same gilts), Student’s t-test was performed. AU arbitrary units.
