Table 1 Used synthetic DNA sequences.
From: New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples
BRCA1 gene mutation: 5370C > T (gene fragment at position c.5251 C > T transition) | |
Without mutation | probe DNA 1 (5′ → 3′): thiol–C6–AGGTCCAAAGCGAGCAAGAGAAGGAGTGGGTCCCATCAGTTTGAA |
target DNA 1 (3′ → 5′): TCCAGGTTTCGCTCGTTCTCTTCCTCACCCAGGGTAGTCAAACTT | |
With mutation | probe DNA 2 (5′ → 3′): thiol–C6–AGGTCCAAAGTGAGCAAGAGAAGGAGTGGGTCCCATCAGTTTGAA |
target DNA 2 (3′ → 5′): TCCAGGTTTCACTCGTTCTCTTCCTCACCCAGGGTAGTCAAACTT | |
BRCA1 gene mutation: 5382insC (gene fragment at position c.5266_67) | |
Without mutation | probe DNA 3 (5′ → 3′): thiol–C6–AAGAGAATCCC☐AGGACAGAAAGGGAGTGGGTCCCATCAGTTTGAA |
target DNA 3 (3′ → 5′): TTCTCTTAGGG☐TCCTGTCTTTCCCTCACCCAGGGTAGTCAAACTT | |
With mutation | probe DNA 4 (5′ → 3′): thiol–C6–AAGAGAATCCCCAGGACAGAAAGGGAGTGGGTCCCATCAGTTTGAA |
target DNA 4 (3′ → 5′): TTCTCTTAGGGGTCCTGTCTTTCCCTCACCCAGGGTAGTCAAACTT | |
BRCA1 gene mutation: c.4035delA (gene fragment at position c.4035) | |
Without mutation | probe DNA 5 (5′ → 3′): thiol–C6–TCAGATGATGAAGAAAGAGGAACGGAGTGGGTCCCATCAGTTTGAA |
target DNA 5 (3′ → 5′): AGTCTACTACTTCTTTCTCCTTGCCTCACCCAGGGTAGTCAAACTT | |
With mutation | probe DNA 6 (5′ → 3′): thiol–C6–TCAGATGATGA☐GAAAGAGGAACGGAGTGGGTCCCATCAGTTTGAA |
target DNA 6 (3′ → 5′): AGTCTACTACT☐CTTTCTCCTTGCCTCACCCAGGGTAGTCAAACTT | |
BRCA1 gene mutation: 185delAG (gene fragment at position c.68_69) | |
Without mutation | probe DNA 7 (5′ → 3′): thiol–C6–CAGAAAATCTTAGAGTGTCCCATGGAGTGGGTCCCATCAGTTTGAA |
target DNA 7 (3′ → 5′): GTCTTTTAGAATCTCACAGGGTACCTCACCCAGGGTAGTCAAACTT | |
With mutation | probe DNA 8 (5′ → 3′): thiol–C6–CAGAAAATCTTAG☐☐TGTCCCATGGAGTGGGTCCCATCAGTTTGAA |
target DNA 8 (3′ → 5′): GTCTTTTAGAATC☐☐ACAGGGTACCTCACCCAGGGTAGTCAAACTT | |
BRCA1 gene mutation: 3819del5GTAAA (gene fragment at position c.3700_3705) | |
Without mutation | probe DNA 9 (5′ → 3′): thiol–C6–ATTTGGTAAAGTAAACAATATACCTGGAGTGGGTCCCATCAGTTTGAA |
target DNA 9 (3′ → 5′): TAAACCATTTCATTTGTTATATGGACCTCACCCAGGGTAGTCAAACTT | |
With mutation | probe DNA 10 (5′ → 3′): thiol–C6–ATTTGGTAAA☐☐☐☐☐CAATATACCTGGAGTGGGTCCCATCAGTTTGAA |
target DNA 10 (3′ → 5′): TAAACCATTT☐☐☐☐☐GTTATATGGACCTCACCCAGGGTAGTCAAACTT | |
