Table 2 List of primers used for PCR amplification. The reference sequence NM_007294.3 was used for the BRCA1.
From: New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples
BRCA1 | Primer name | Primer sequence (5′ → 3′) | Amplicon size |
|---|---|---|---|
Exon 2 | BRCA1_ek2_F_HRM | GAAGTTGTCATTTTATAAACCTTT | 258 bp |
BRCA1_ek2_R_HRM | TGTCTTTTCTTCCCTAGTATGT | ||
Exon 4 | BRCA1_ek5_F_HRM | CTCTTAAGGGCAGTTGTGAG | 235 bp |
BRCA1_ek5_R_HRM | TTCCTACTGTGGTTGCTTCC | ||
Exon 10 | BRCA1_ek11_F_HRM | CAGGGAGTTGGTCTGAGTGAC | 181 bp |
BRCA1_ek11_R_HRM | GCTCCCCAAAAGCATAAAC | ||
Exon 19 | BRCA1_ek20_F_HRM | ATATGACGTGTCTGCTCCAC | 401 bp |
BRCA1_ek20_R_HRM | GGGAATCCAAATTACACAGC |
