Figure 9
Effects of the Aplysia elevenin analogs with alanine substitution on the three putative receptors. (A-F) Representative examples of dose–response curves of the activation of apElevR1, apElevR2, and apElevR3 with substitution of a conserved amino acid of elevenin with ala. (G-I) The log[EC50] values of apElevR1, apElevR2, and apElevR3 after replacing different conserved amino acids of elevenin with ala (most conserved amino acids included I4, D5, F11, P13, R16, G17, and V18 as predicted by Weblogo v2.8.2), One-way ANOVA, F (7, 16) = 80.34, P < 0.0001 (G), F (5, 12) = 88.51, P < 0.0001 (H), F (7, 16) = 55.08, P < 0.0001 (I). Bonferroni post-hoc test: *P < 0.05, **P < 0.01, ****P < 0.0001. Error bar: mean ± SEM. (J) Sequences of all peptides tested and a summary of the average log[EC50] and EC50 values on apElevRs. n = 3 for all the tests. Cysteines in red represent the disulfide bond in the peptide. Alanine in orange denotes the substitution of each residue in the peptide analogs. Analogs with substituted residues inside the disulfide bond were highlighted in blue.
