Figure 2
From: Optimized metrics for orthogonal combinatorial CRISPR screens
CHyMErA performance is dependent on enAsCas12a gRNA processing activity. (a) Comparison of SpCas9 and enAsCas12a gRNA performances from CHyMErA screen. Each data point represents SpCas9 (gRNA-NHT) vs. enAsCas12a (NHT-gRNA) LFC values. (b) LFC comparison of SpCas9 gRNAs from SpCas9 and CHyMErA screens, and (c) enAsCas12a gRNAs from enAsCas12a and CHyMErA screens. Each data point represents a gRNA expressed from the hU6 promoter. (d) Gene level LFC comparison of SpCas9 single gRNAs and enAsCas12a single gRNAs within CHyMErA screens. (e) Gene level LFC comparison of SpCas9 single gRNAs within SpCas9 (gRNA:NHT pairs) and CHyMErA (gRNA:NHT pairs) screens. (f) Gene level LFC comparison of enAsCas12a single gRNAs within enAsCas12a (gRNA:NHT pairs) and CHyMErA (gRNA:NHT pairs) screens. NHT-CE gRNA pairs targeting a CE gene are highlighted in pink, and NHT-TS gRNA pairs targeting a TS gene in blue. (g) Cell proliferation analysis of cells that were transduced with TP53 and HPRT1 targeting sgRNAs or dual-gRNAs. Cells were treated either with DMSO (left panel) or Nutlin3 + 6TG (right panel). The shaded area shows the statistical difference between the curves. Data are means of replicates (n = 3). (d-f) The linear regression is shown as a dashed line.
