Figure 5
From: Optimized metrics for orthogonal combinatorial CRISPR screens
The effect size range of the multiSPAS is larger with enAsCas12a (a) Schematic representation of enAsCas12a and opAsCas12a nuclease expression constructs and scheme of the multiSPAS gRNA expression vector. (b) Comparison of gene level LFC (top) and gRNA level LFC (bottom) for multiSPAS(enAsCas12a) and multiSPAS(opAsCas12a). Lines represent the median LFC value. (c) Scatter plot comparing LFC of CE, TS, NHT-CE/TS gene pairs from multiSPAS(enAsCas12a) and multiSPAS(opAsCas12a) screens. CE-CE pairs targeting two CE genes are labeled with pink, TS-TS pairs targeting two TS genes with blue, and NHT-CE and NHT-TS pairs targeting a single gene with green and gray respectively. (d) Comparison of CE and TS gene level LFC for SpCas9 gRNAs and (e) AsCas12a (all gRNAs in the library) gRNAs from multiSPAS(enAsCas12a) and multiSPAS(opAsCas12a) screens. (f) Gene level LFC comparison of TTTV PAM-AsCas12a gRNAs from multiSPAS(enAsCas12a) and multiSPAS(opAsCas12a) screens. (d–f) NHT-CE gRNA pairs targeting a CE gene are highlighted in pink, and NHT-TS gRNA pairs targeting a TS gene are in blue. The dashed line is the linear regression line.
