Figure 1 | Scientific Reports

Figure 1

From: The cytoplasmic N-terminal tail of Zika virus NS4A protein forms oligomers in the absence of detergent or lipids

Figure 1

Purification of ZIKV NS4A ZN peptide. (A) Sequence of purifed ZN peptide, with His tag (black), linker (red) and residues 4–58 of NS4A (blue). The sequence of truncated ZN′ is also shown; (B) NuPAGE of the Ni–NTA fractions, where LYS, FT, Wash, and Elu are lysate, flow-through, wash and elution from the IMAC column, respectively. The white arrowhead points to the ZN peptide band; (C) HPLC chromatogram of ZN peptide; (D) NuPAGE gel image of the HPLC fractions in (C); (E) MALDI-MS spectra of the fractions in (C) with molecular weights indicated; (F) schematic representation of ZIKV NS4A, where the topology and helical domains are assumed to be the same as in NS4A DENV20,24. The N-terminal peptide ZN (4–58) used in the present work is indicated as a dotted line. Gels in panels (B) and (D) were cropped from complete gels shown in Supplementary Fig. S1. The illustration in (F) has been made in Adobe Illustrator.

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