Figure 5
From: Hedgehog morphogen gradient is robust towards variations in tissue morphology in Drosophila
Inducing an ectopic fold at the PA boundary of the wing disc does not affect the slope of the Hedgehog gradient. (a) Schematic representation of a wing disc showing the Ptc-expression domain (identifying the PA boundary). (b) Schematics of the side view of the wing disc for wild-type (top) and ptc-Cad86C (bottom). Cell outlines and Ptc domain are shown. (c) y-projection (mean) of regions of interest in wild-type and ptc-Cad86C wing discs immunostained for Ptc and E-cadherin (left) and Hh (right). Scale bars are 20 μm. (d–e) Normalized Hh fluorescence relative to the contour length (PA boundary set as 0) for N = 10 wild-type and N = 10 ptc-Cad86C wing discs. Curves and single exponential fits are shown. Light grey box indicates the range of contour length corresponding to the fold (average). Darker grey box indicates the range of the fold center. (f) Decay rate extracted by the fits in panels (d) and (e). P-value obtained from a two-sample t-test.
