Figure 3
Subcellular localization of Hic-5 in human chondrocytes stimulated by mechanical stress. (A) Intracellular localization of Hic-5 in human chondrocytes exposed to mechanical stress (MS+) for 30 min or 3 h, or untreated (MS−). Arrow heads indicate focal adhesions. (B) Immunofluorescence imaging of nuclear Hic-5 in human chondrocytes after mechanical stress. Human chondrocytes were exposed to 10 ng/ml leptomycin B (LMB) and mechanical stress for 1 h. Nuclei were counterstained with DAPI (blue). Representative image was selected from 3 biological replicates. Original magnification: × 400. Bar = 50 μm.
