Table 1 Distribution of the Ct values of clinical saliva samples used for pooling.

From: Pooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2

Ct range*

No. samples workflow A (n = 22)

No. samples workflows B–E (n = 25)

20.0–29.9

7 (32%)

7 (28%)

30.0–34.9

5 (23%)

8 (32%)

35.0–40.0**

10 (45%)

10 (40%)

  1. *Samples < 40 Ct are considered positive for SARS-CoV-2.
  2. **Mean Ct for the CFX96 Touch RT-qPCR instrument when determining LOD for analytical sensitivity using this set of reagents was 36.7.
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