Figure 2

Isolation of the B. pertussis Agg^- mutant from the Agg⁺ strain. (A) Autoagglutination assay of BP300 and BP300s cultured on CSM plates. (B) Autoagglutination was visualized in test tubes after 5 h of static incubation (upper panel). Phase-contrast micrographs after 5 h of static incubation (lower panel). Magnification×100. (C) Single-point mutation detected in the fim3 promoter region of Agg^- mutant BP300s. The upstream sequences of fim3 are shown and the SNP position is highlighted in gray. The -35, -10, and + 1 positions of the Pfim3 region are indicated based on a previous publication⁷. (D) Immunoblot analysis using anti-Fim3 antibody.