Table 1 Accuracy and precision for tNGF (pg/mL) during assay qualification.

From: Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

tNGF

Level

QC1

QC2

QC3

QC4

Nominal

½ Endogenous

Endogenous

Endogenous + 45 pg/mL

Endogenous + 450 pg/mL

Target tNGF level (pg/mL)

15.0

30.0

75.0

485

Qual Run

1

2

3

1

2

3

1

2

3

1

2

3

Rep 1

9.92*

12.8

11.9

32.0

31.9

27.5

65.0

59.6

62.8

475

485

483

Rep 2

14.1

10.1*

14.6

32.0

30.3

27.6

74.2

60.8

67.4

434

380

524

Rep 3

15.8

11.0*

13.0

27.8

25.6

26.1

62.9

73.2

77.4

458

418

465

Rep 4

12.1

13.2

14.1

33.0

30.1

26.6

63.9

60.3

71.3

426

406

487

Rep 5

11.1*

14.9

10.5*

34.5

35.1

30.5

73.9

68.9

85.0

420

405

440

Rep 6

15.6

12.4

12.5

26.0

32.5

30.9

71.1

74.8

90.9

432

375

488

Avg

13.1

12.4

12.8

30.9

30.9

28.2

68.5

66.3

75.8

440.8

411.5

481.2

SD

2.44

1.69

1.49

3.27

3.17

2.02

5.16

6.90

10.72

21.17

39.60

27.84

CV %

18.6

13.6

11.7

10.6

10.3

7.16

7.53

10.4

14.1

4.80

9.62

5.79

RE %

−12.6

17.3

14.9

2.94

3.06

−6.00

−8.67

11.6

1.07

2.04

−8.56

6.93

Inter-Assay

Mean

12.8

30.0

70.2

445

SD

1.82

3.00

8.56

41.0

CV %

14.3

10.0

12.2

9.23

RE %

−150

NA

−6.41

−7.40

  1. All points included in statistical calculations.
  2. Qual qualification, Rep replicate, Avg average, SD standard deviation, RE relative error.
  3. *Accuracy > 25%.
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