Figure 4 | Scientific Reports

Figure 4

From: Proof of principle study: synchrotron X-ray fluorescence microscopy for identification of previously radioactive microparticles and elemental mapping of FFPE tissues

Figure 4The alternative text for this image may have been generated using AI.

BNP was used to identify Si-containing microparticles in the lymph node from ROI2 (defined in Fig. 1b) using high-resolution XFM (80 nm X-ray beam spot size). Elemental mapping (a) and spectrum of Kα X-rays produced by the sample (b) detects Si, P, S, Fe, and Zn in 289,081-pixel images. Three color colocalization of Si, P and Fe signals (c) displays Si signals that may correspond to potential aggregates of aluminosilicate particles and single aluminosilicate particles (P1 and P2). Particle size distribution analysis of aluminosilicate particles generated at ITRI (d) are based on the publication by Rabbe and others39. Approximate diameters of P1 and P2 measured using MAPS software (c) fall within the particle size distribution of aluminosilicate particles. Elemental ROI analysis of P1 and P2 shows the relative elemental concentrations of Si, P, Fe, and Zn in Si-rich (ROI1) and neighboring Si-poor (ROI2) regions of similar size (Methods). Small regions with overall low signals serve as background for subtraction (Background ROI). Si-rich regions no longer correlate with P and Fe in the same way as they did at medium resolution. Color bar in (a) shows per pixel signal intensity going from low (black) to medium (blue) to high (white). Scale bar in (a) is 20 μm.

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