Table 1 List of primers for mus musculus used in this study.

From: Nicotinamide N-methyltransferase (NNMT) regulates the glucocorticoid signaling pathway during the early phase of adipogenesis

Primer name

Forward sequence (5′–3′)

Reverse sequence (5′–3′)

Real-time PCR gene expression

Gapdh

GTGCAGTGCCAGCCTCGTCC

GCCACTGCAAATGGCAGCCC

Nnmt

CTTTGGGTCCAGACACTGTGCA

CCAGAGCCAATGTCAATCAGGAG

Cebpb

GGTTTCGGGACTTGATGCA

CAACAACCCCGCAGGAAC

Cebpa

TGGACAAGAACAGCAACGAGTAC

GCAGTTGCCCATGGCCTTGAC

qPCR ChIP

CEBPB Binding Site 1 (CBS1)

AAATCACTCTGTAGTCCAGGCTGT

GACTCCAAAAGCTAACTTCACAGG

CEBPB Binding Site 2 (CBS2)

AGATTCCATCGTGTCTCAGCTC

TCTAAGACTCCAAAAGCCAACTTC

CEBPB Binding Site 3 (CBS3)

AAGTTGGCTTTTGGAGTCTTAGAG

ACGTCTGCAGTCTATTTCACTGTC

CEBPB Binding Site 4 (CBS4)

CAGTGAAATAGACTGCAGACGTTC

ACTCCCCTCTTCTCCTAAGCTC

CEBPB Binding Site 5 (CBS5)

TCATCTCCAAACAATCCAGTAGTC

TTGTTTGTTCAAACTGAAACCATT

CEBPB Binding Site 6 (CBS6)

AGAAAAGGGAATGTGGGGTAAG

CAGACTCCATCCGAGATTATTTTT

CEBPB Binding Site 7 (CBS7)

TTTAAAACATGGACATTGTCTTCC

CTCATTGAGTCCTAGTGGTGCTG

CEBPB Binding Site 8 (CBS8)

CTTCACAGAGTTGTTGCTCAAAAT

GGAGATTCTCTGGGTGAGTTTTTA

Luciferase reporter vector

Nnmt-full promoter

CGATCTAAGTAAGCTGAGACGGGGTCTAACTATGTAACTG

CCGGAATGCCAAGCTCGTGTCTCAGCTCCGTGC

Nnmt-mut CBS4

CAGTGAAATAGACTGCAGACGTTCAGGCTACGTAAGACAGTGATGAAATTCCTGGAGAAGGGAGCTTA

TAAGCTCCCTTCTCCAGGAATTTCATCACTGTCTTACGTAGCCTGAACGTCTGCAGTCTATTTCACTG

Nnmt-mut CBS8

TTGATTAACCAGCCGGAGATGGGTAACACAATGCGAAAGCAGTAAGTTCCTGGCATCCCACG

CGTGGGATGCCAGGAACTTACTGCTTTCGCATTGTGTTACCCATCTCCGGCTGGTTAATCAA

CRISPR gRNAs vector

Nnmt-Guide1

CTTGTGGAAAGGACGAAACACCGGGAGAACTCCTGATTGACATGTTTTAGAGCTAGAAA

TTTCTAGCTCTAAAACATGTCAATCAGGAGTTCTCCCGGTGTTTCGTCCTTTCCACAAG

Nnmt- Guide 2

CTTGTGGAAAGGACGAAACACCGGGGGACCAGTCAAAGGCTCCGTTTTAGAGCTAGAAA

TTTCTAGCTCTAAAACGGAGCCTTTGACTGGTCCCCCGGTGTTTCGTCCTTTCCACAAG

Sanger sequencing

Nnmt-seq

CCTTCTTCAGCCATTTCTGC

TGCAGGTCCCTTCAGAAAGT

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