Figure 1 | Scientific Reports

Figure 1

From: Single cell profiling at the maternal–fetal interface reveals a deficiency of PD-L1+ non-immune cells in human spontaneous preterm labor

Figure 1The alternative text for this image may have been generated using AI.

Experimental design and workflow. (A) Preparing decidual tissues for single cell analysis on the CyTOF platform. Our study included tissues from 16 term laboring, 12 term non-laboring, 11 preterm laboring, and 14 preterm non-laboring pregnancies (see Table 1). See Supplementary Methods for antibody staining with 32-antibody panel and CyTOF analyses. (B) After data processing and QC, the cells were first divided into CD45+ (immune) and CD45- (non-immune) pools, then characterized using two additional filters for hierarchical SNE analysis. (C) The first filter identified six immune cell types and six non-immune cell populations by 14 and 11 markers, respectively. (D) The second filter identified 10 T cell subsets, five macrophage/monocyte subsets, and two granulocyte subsets. L laboring, NL non-laboring. DCs Dendritic cells, DSC Decidual stromal cell, EVT extravillous trophoblast. Undefined: No cell-surface markers to identify the cell type.

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