Figure 2
From: Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
MiR-183 targets PTEN expression. (A) PTEN is a direct target of miR-183 demonstrated by 3UTR luciferase assay; *p = 0.0451; Wnt-7b gene 3′UTR sequence was used as off target in this experiment. (B) The transfection efficiency of miR-183 was measured by qRT-PCR 48 h after transfection **p = 0.0032, **p = 0.0012 (C) The mRNA of PTEN was measured by Real-time PCR in MCF-7 and MDA-MB-231 transfected with miR-183; *p = 0.0167, *p = 0.0200 (D) The protein expression of PTEN was measured by Western blot assay in MCF-7 and MDA-MB-231 transfected with miR-183; *p = 0.0273, *p = 0.0420. A full-length image is included in a Supplementary Information file. NC negative control. *p < 0.05; **p < 0.01; ***p < 0.001.
