Table 1 Primers used in this study.
From: Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
Primers | Sequence of primers |
|---|---|
miR-183-F | 5-GACTATGGCACTGGTAGAATTCA-3 |
Universal | 5-GCGTCGACTAGTACAACTCAAG-3 |
U48-F | 5-TGATGACCCCAGGTAACTCTG-3 |
Universal | 5-GCGTCGACTAGTACAACTCAAG-3 |
PTEN-F | 5-CACACGACGGGAAGACAAGT-3 |
PTEN-R | 5-TCCTCTGGTCCTGGTATGAAG-3 |
GAPDH-F | 5-ACCCACTCCTCCACCTTTGAC-3 |
GAPDH-R | 5-TGTTGCTGTAGCCAAATTCGTT-3 |
PTEN-3UTR-XhoI-F | 5-AATTATCTCGAGAGAATTTGACAAGAATTGCTATGAC-3 |
PTEN-3UTR-NotI-R | 5-AATTAAGCGGCCGCGTCCCACTGTAACATTGTCTACT-3 |
Pre-miR-183- XhoI-F | 5-AATTATCTCGAGCATGTGGATCTTGTGAAGAGGTG-3 |
Pre-miR-183-HindIII-R | 5-AATTAAAAGCTTCTTGCATCCCTGCACCCTTG-3 |
