Figure 1

Top—kinetics of the binding reaction between peptides mimicking the C-terminal region of the Envelope proteins from SARS-CoV, SARS-CoV-2 and MERS-CoV versus different concentrations of PDZ2 domain of ZO1. Straight lines are the best fit to a linear equation. R2 values for linear regression are reported. Bottom—as described in the text, points at 0 concentration of ligand were measured in separated displacement experiments. Lines are the best fit to a single exponential equation.