Figure 2
From: Novel genetically engineered mouse models for clear cell renal cell carcinoma
Organ- and sequence specific induction of mutations in Bap1 and Pbrm1 in BPS-TA mice. (A) Mutation frequencies in kidneys, liver, spleen, heart, lung, intestine, testis, and muscle of BPS-TA mice. Kidneys from WT and TRACK mice provided negative controls, while the intestines from dox-treated transgenic founder animals (BPS-R26/rtTA) served as positive control for genome editing. Pbrm1 and Bap1 are shown at the top and the bottom, respectively. (B) Mutation frequencies in different regions of BPS-TA kidneys. Livers from BPS-TA mice and WT kidneys provided negative controls, while the intestines from dox-treated transgenic founder animals (BPS-R26/rtTA) served as positive controls for genome editing. Overall morphology and histology of a BPS-TA kidney and the different regions selected for analysis are shown at the top. Pbrm1 and Bap1 are shown at the bottom left and the bottom right, respectively. Note that all BPS-TA mice evaluated were biallelic for each of the three transgenes. The images and figure were compiled in Adobe Illustrator software.
