Figure 4
From: Proteomic analysis of the effect of hemin in breast cancer
Hemin induces the proteases involved in HO-1 cleavage (A) LFQ intensity levels profile of calpain 1 and cathepsin B. (B) Western blot shows cathepsin B protein expression. GAPDH was used as loading control. Bottom: Densitometric quantification of HO-1/actin ratio, (***p < 0.001; Student's T-test). (C) Western blot shows native (32 kDa) and truncated (28 kDa) HO-1 protein expression after hemin treatment. Actin was used as loading control. Bottom: Densitometric quantification of HO-1/actin ratio (n = 3), (*p < 0.05; Student's T-test).
