Table 2 Kinetic constants of PzaA.

From: Identification of a mycobacterial hydrazidase, an isoniazid-hydrolyzing enzyme

Substrate

Vmax

Km

Vmax/Km

(μmol min−1 mg−1)

(mM)

(μmol min−1 mg-1 mM−1)

Isoniazid

1.51 (0.140)

1.20 (0.271)

1.26

Nicotinic acid hydrazide

23.7 (0.788)

1.26 (0.0475)

18.8

Isonicotinamide

6.48 (0.102)

0.145 (0.0203)

44.7

Nicotinamide

37.2 (0.287)

0.0887 (0.0113)

419

Pyrazinamide

11.7 (0.508)

0.143 (0.00780)

81.8

  1. Enzyme assays with hydrazides and amides as substrates were performed using colorimetric methods that monitored the formation of hydrazine and ammonia, respectively. Lineweaver–Burk plots were used to calculate the Vmax and Km values. Data represent means of triplicate values obtained from three separate data sets. Standard deviations are shown in parentheses.
Back to article page