Table 3 Correspondence between culture growth and the organisms detected by next-generation sequencing.

From: Direct detection and identification of periprosthetic joint infection pathogens by metagenomic next-generation sequencing

Organisms

Culture (N = 36)

mNGS (N = 59)

Culture vs mNGS (DT + SF)

DT*

SF*

DT*

SF*

Staphylococcus aureus

14

16

14

15

16/15

Staphylococcus epidermidis

8

9

8

9

9/9

Escherichia coli

5

3

8

7

5/8

Enterobacter cloacae

2

1

3

4

2/5

Enterococcus faecalis

2

1

2

2

2/3

Streptococcus sanguinis

1

0

0

0

1/0

Klebsiella pneumoniae

1

0

2

2

1/3

Candida albicans

1

1

1

1

1/1

Pseudomonas aeruginosa

0

0

4

3

0/4

Cutibacterium acnes

0

0

1

2

0/3

Staphylococcus lentus

0

0

2

1

0/2

Streptococcus pneumoniae

0

0

2

1

0/2

Serratia marcescens

0

0

1

2

0/2

Aspergillus flavus

0

0

1

0

0/1

Staphylococcus warneri

0

0

1

1

0/1

Staphylococcus capitis

0

0

1

1

0/1

Streptococcus lactis

0

0

1

1

0/1

Staphylococcus haemolyticus

0

0

1

1

0/1

Staphylococcus cohnii

0

0

1

1

0/1

Enterobacter hormaechei

0

0

0

1

0/1

Finegoldia magna

0

0

1

1

0/1

Anaerococcus prevotii

0

0

1

1

0/1

Pseudomonas oleovorans

0

0

0

1

0/1

Corynebacterium kutscher

0

0

1

1

0/1

Streptococcus mitis

0

0

1

1

0/1

Mycobacterium tuberculosis

0

0

1

1

0/1

  1. mNGS metagenomic next-generation sequencing, DT deep tissue, SF synovial fluid.
  2. *The values are given as the detected frequency of a corresponding organism in each single types of specimen.
  3. The values are given as the detected frequency of a corresponding organism in all types of specimen by culture versus the detected frequency of a corresponding organism in all types of specimen by mNGS.